Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena.
نویسندگان
چکیده
A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 70 9 شماره
صفحات -
تاریخ انتشار 2006